Journal: The ocular surface
Article Title: Aqueous humor induces lymphatic regression on the ocular surface
doi: 10.1016/j.jtos.2020.03.002
Figure Lengend Snippet: Aqueous humor induced the regression of lymphatic tubes formed by human microdermal lymphatic endothelial cells in vitro. (A) Representative images showing lymphatic meshes 5 hours after culturing with AH or control medium, respectively. Scale bar: 100 μm. Images showing the sites of regression, as indicated by the arrows, are presented in (B). Scale bar: 20 μm. (C) Summarized data assessed in the number and mean size of the meshes, respectively. Assays were performed in triplicates and repeated once. *p < 0.05.
Article Snippet: Briefly, human neonatal microdermal LECs were purchased from AngioBio (AngioBio Co., Del Mar, CA, USA), and maintained in EBM MV2 growth medium (Promocell, Heidelberg, Germany), supplemented with 10% bovine calf serum (HyClone, Logan, Utah) and 1% penicillin/streptomycin.
Techniques: In Vitro, Control